At that time, the early symptoms were dismissed as particles, nevertheless the retrospective analysis demonstrated shared properties With all the problematic cells in culture. Tracing the infection helped the lab set up added laboratory treatments for screening and protection of assay-essential cell traces.
As samples are obtained to the Attune CytPix Flow Cytometer, the higher-pace brightfield camera captures and retailers photographs of detected activities in a price as much as 6,000 illustrations or photos for each 2nd, according to the flow amount and image dimension. For larger versatility, Attune Cytometric Software permits you to change the image capture frequency as needed and select a certain gate for impression capture, if demanded.
Blend acoustic concentrating technology with high-parameter Evaluation for flexible spectral unmixing and standard workflows.
Discover new insights into your experimental facts by visualizing populations utilizing put together fluorescence and morphology details. Our computer software interface wizards help downsampling, concatenation (merged files) and dimensionality applications which can be designed into the software for an intuitive, streamlined information Assessment practical experience.
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Below, a qualified person has gated singlets confidently. Just after analyzing the guide singlet gate, the CytPix graphic-derived parameter ParticleCount reveals this gate has over four% aggregates.
Optimizing gating strategy. Even strong manual singlet gating is error-susceptible and stays a subjective decision place in Pretty much all flow cytometry assays. Imaging can be employed to substantiate and adjust gates to incorporate only one cells of curiosity.
Constant image top quality even at higher throughput Acoustic focusing and a higher-velocity digital camera combine to graphic these T cells continuously at low or higher flow charges. Very easily regulate focus and digital camera settings to satisfy experimental requirements.
Early indications of this contamination experienced to begin with been dismissed as particles. Backgating to find out new populations. Backgating imaged cells to the Attune CytPix Flow Cytometer also allows you to use morphological capabilities to find out exciting subpopulations that may not be obvious from flow cytometry details alone.
End users can perspective function pictures in authentic-time to verify sample high quality, enhance protocols, and document the morphology of each and every mobile population determined for Evaluation. Imaging seize also Positive aspects from acoustic concentrating, which helps to placement the cells so that a sharp, centered graphic is obtained.
AI-driven automatic impression Assessment Together with the Attune Cytometric Software program interprets celebration options into distinct morphology parameters that can be combined with normal fluorescence and scatter parameters to reveal hugely reproducible population alterations and one of a kind subsets.
Software Observe: Automated graphic Investigation lessens read more user-to-user variability in flow cytometry gating tactics
Leverage the power of Innovative technological know-how and innovation to speed up your discoveries. Believe in within the Attune Flow Cytometer family members to deliver the reliability, speed, and accuracy that set your do the job apart.
Adaptable payment plan from Agilent Monetary Answers permit you to get the significant instrumentation you may need for instant venture, or to develop your lab.
Gating on these prolonged parameters enables you to promptly and accurately establish populations of fascination and confirm gating tactic with little or no manual evaluation. The Attune application presents use of point out-of-the-artwork device Mastering Assessment tools in just an expertly built user interface, which makes it extremely convenient to use.
As the mobile line had Beforehand been analyzed to the Attune CytPix Flow Cytometer, the scientists went again to the photographs and were being able to document the microbial an infection at least 5 times earlier.
Agilent Price Assure assures you at the very least a decade of instrument use from your day you buy. Usually, We are going to credit you With all the residual price of the technique towards an upgraded design.
What appeared to be a homogeneous populace is in fact much more diverse—an insight that should be regarded as when interpreting results.